Human Anti-Mouse Antibodies, HAMA

Immunology

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Description

Human anti-mouse antibodies (HAMA) are human immunoglobulin with specificity for mouse immunoglobulin [1]. Mouse antibodies and their fragments are used in various diagnostic procedures, such as in radiolabelled reagents for imaging studies [2] and in laboratory test reagents [3,4]. They are increasingly being used in biological treatments for patients (eg. rituximab), particularly in those with autoimmune disease [5]. A single dose of mouse monoclonal antibodies has the potential to induce an immune response initiating the production on anti-mouse antibodies. However it has been shown that the concentration and IgG isotype of murine antibody used does not influence the production of HAMA [2]. Circulating HAMA has the capability to bind to mouse antibodies that are used in subsequent procedures or treatments. This diminishes the efficacy of the antibody based treatments. The presence of HAMA in patient samples can also be a cause of false positive or false negative immunoassay results, depending on the assay principles and the type of monoclonal antibodies used in the test [3,4,6].

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Indications

The detection of Human Anti-Mouse antibodies.

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Sample Type

2mL Fresh Serum (Gel 5mL Yellow tube). Transport fresh or freeze if the sample is not posted within 48 hours of being collected. Requests from outside Sheffield: Transport at ambient temperature via Royal Mail or Courier.

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Reference Range

<15 ng/mL indicates absence of Human anti-mouse antibodies (HAMA). Reference range verified

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Turnaround Time

Within 6 months

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Testing Frequency

As required

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References

Klee GG. Human anti-mouse antibodies. Archives of Pathology and Laboratory Medicine. 2000, 124; 921-923. [Ref 1].Reynolds JC, Del Vecchio S, Sakahara, et al. Anti-Murine antibody response to mouse monoclonal antibodies: Clinical findingd and implications. Int J Radiat Appl Instrum Part B: Nuclear Medicine and Biology. 1989. 16; 121-125. [Ref 2].Kricka LJ. Human anti-mouse antibody interferences in immunological assays. Clin Chem. 1999. 45; 942-956. [Ref 3].Siddiqui MZ. Monoclonal antibodies as diagnostics: An appraisal. Indian J Pharm Sci. 2010. 72; 12-17. [Ref 4].Senolt L, Vencovsky J, Pavelka K, et al. Prospective new biological therapies for Rheumatoid arthritis. Autoimmunity reviews. 2009. 9; 102-107. [Ref 5].Sargur R, Cowley D, Murng S, et al. Raised tryptase without anaphylaxis or mastocytosis: heterophillic antibody interference in the serum tryptase assay. Clin Exp Immunol. 2011. 163; 339-345. [Ref 6].

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