Eosinophil Cationic Protein (ECP)

Immunology

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Description

ECP is a highly cytotoxic protein found in eosinophil granules. Eosinophils are the cells chiefly responsible for producing the inflammation characteristic of asthma. When eosinophils in the airway are activated, they undergo degranulation causing airway epithelial damage and desquamation. This can increase hypersensitivity and lead to chronic inflammatory disease of the airway. ECP is released from the activated eosinophils during the inflammation process [2]. Serum ECP levels have been shown to correlate with ECP concentrations in the bronch-alveolar lavage fluid, which in turn correlates to the severity of asthma [1]. However ECP can also be raised in atopic conditions such as allergic rhinitis [2].

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Indications

Eosinophilic inflammation (e.g. asthma).

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Sample Type

2mL Serum (Gel 5mL Yellow tube). Requests from outside Sheffield: Transport at ambient temperature via Royal Mail or Courier.

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Reference Range

Normal range 1.0 - 15.0 ug/L.

Reference range manufacturer derived and verified in-house.

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Turnaround Time

Within 5 days

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Testing Frequency

As required

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External Notes

Serial samples should be taken at the same ambient temperature to minimise variability induced by artefactual release of ECP by eosinophil breakdown, which may be accelerated at higher temperatures.

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References

PRU handbook of clinical immunochemistry. 9th Edition. 2007. [Ref 1]
Koh G, et al. Eosinophil cationic protein: Is it useful in asthma? A systematic review. Resp Med. 2007. 101:696-705. [Ref 2]
Venge P, et al. Eosinophil cationic protein (ECP): molecular and biological properties and the use of ECP as a marker of eosinophil activation in disease. Clin Exp Allergy. 1999. 29(9):1172-1186.

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Please note: the above information is subject to change and we endeavour to keep this website up to date wherever necessary.