Glucose-6-Phosphate Dehydrogenase ( G6PD )

Haematology

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Description

G6PD catalyses the first step in the pentose phosphate pathway, oxidising glucose-6-phosphate ( G6P ) to 6-phosphogluconate ( 6PG ) and reducing nicotinamide adenine dinucleotide phosphate ( NADP ) to reduced NADPH. The rate of formation of NADPH is proportional to the G6PD activity,and is measured by the change in absorbance at 340nm.

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Indications

F/H of deficiency. Prior to treatment of Vivax Malaria.In all cases, a screening test will be performed as the initial investigation unless screening has already been performed by a referring laboratory.

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Sample Type

Whole blood ( EDTA 4.0ml Lavender )

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Reference Range

Screen will be reported as Normal or Deficient
Normal range for Assay = 4.6 - 13.5 IU/gHb

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Turnaround Time

Within 1 day

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Testing Frequency

As Required

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External Notes

Reticulocytes have a higher G6PD level than mature red cells.  G6PD may therefore be falsely elevated after a severe haemolytic crisis or any other situation where a reticulocytosis exists. Samples from neonates will have a higher value of G6PD. In neonates with Normal G6PD levels the comment  ‘Please note that G6PD levels may be falsely normalised in neonates. Suggest repeat at 1 year of age if indicated by a strong family history of G6PD deficiency.

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References

Trinity Biotech Kit 203-A G6PDH Screening test insertTrinity Biotech Kit 345-B G6PDH Assay test insert

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Please note: the above information is subject to change and we endeavour to keep this website up to date wherever necessary.