Mitochondrial Antibody, PBC Screen (AMA)

Immunology


Description

The liver antibody screen utilises a composite tissue block of mouse liver kidney and stomach to identify anti-nuclear antibodies, gastric parietal cell antibodies, anti- mitochondrial cell antibodies and liver kidney microsomal antibodies all of which can be used to assess liver disease. Anti-Mitochondrial Antibodies (AMA) are strongly associated with Primary Biliary Cirrhosis (PBC), although they can be seen in other autoimmune conditions such as rheumatoid arthritis, systemic sclerosis and chronic liver disease [1]. Different types of AMA are described, based on antigen specificity, designated on a numeric basis: M1 to M9. The AMA associated with PBC is usually M2a or M2b [1]. Not all can be typed by IIF.


Indications

Primary Biliary Cirrhosis (PBC).


Sample Type

2mL Serum (Gel 5mL Yellow tube). Samples must be urgently transported to the laboratory. Requests from outside Sheffield: Please discuss transport requirements prior to requesting this test.


Reference Range

Normal result = Negative.


Turnaround Time

Within 5 days


Testing Frequency

Twice weekly


References

Jones DE. Pathogenesis of primary biliary cirrhosis. Clinics in liver disease. 2008. 12(2):305-321.
Bogdanos DP, Invernizzi P, Mackay IR. Autoimmune liver serology: current diagnostic and clinical challenges. World Journal of Gastroenterology. 2008. 14(21):3374-3387.
Muratori L, et al. Antimitochondrial antibodies and other antibodies in primary biliary cirrhosis; diagnostic and prognostic value. 2008. 12(2):261-276.
PRU Handbook of autoimmunity. 4th Edition. 2007. [Ref 1]
Wesierska-Gadek J, et al. Correlation of initial autoantibody profile and clinical outcome in primary biliary cirrhosis. Hepatology. 2006. 43(5):1135-1144.
Czaja AJ. Autoantibodies in autoimmune liver disease. Advance in Clinical Chemistry. 2005. 40:127-164.


See Also

ANA; SMA; GPC

Please note: the above information is subject to change and we endeavour to keep this website up to date wherever necessary.

Your contact for this test

team

Clare Del-Duca BSc (Hons) Biomedical Science, MSc Pathological Science

Laboratory Manager - Immunology and Protein Reference Unit

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Mitochondrial Antibody, PBC Screen (AMA)