Routine Sickle Test

Haematology

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Description

When HbS is present in the reduced form it is insoluble in a phosphate buffer. The test involves adding a small volume of whole blood to a phosphate buffer containing sodium dithionite as a reducing agent and saponin as a lysing agent. As HbS is less soluble than normal adult haemoglobin in the presence of the reducing agent the solution remains cloudy whilst in normal patients the solution becomes clear.

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Indications

A sickle solubility test will be performed in all instances where the primary Haemoglobinopathy screening method (Capillary Electrophoresis) detects a structural Hb variant.

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Sample Type

Whole Blood (EDTA 4ml Lavender top)

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Reference Range

N/A. Result is either Positive or Negative.

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Turnaround Time

Within 2 days

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Testing Frequency

Daily

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External Notes

The Sickle test is not available in isolation and a full haemoglobinopathy work-up will follow (see HOP/THAL/SICKLE). Please use the ICE code 'HOP' when ordering.

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Please note: the above information is subject to change and we endeavour to keep this website up to date wherever necessary.