Sickle Cell Screen (Pre op)

Haematology

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Description

When HbS is present in the reduced form it is insoluble in a phosphate buffer. The test involves adding a small volume of whole blood to a phosphate buffer containing sodium dithionite as a reducing agent and saponin as a lysing agent. As HbS is less soluble than normal adult haemoglobin in the presence of the reducing agent the solution remains cloudy whilst in normal patients the solution becomes clear.

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Indications

Pre-anaesthetic/pre-op in appropriate ethnic groups.

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Sample Type

Whole blood ( EDTA 4.0ml Lavender )

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Reference Range

Not appliacle. Test result will be Positive or Negative

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Turnaround Time

Within 1 day

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Testing Frequency

Daily

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External Notes

The Sickle test is not available in isolation and a full haemoglobinopathy work-up will follow ( see HOP/THAL/SICKLE ).

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References

TCS Biosciences Sickle-Check kit insert. Doc No. SC/ 4/2013 Based on the work of ITANO, Arch. Biochem 47,148-159.

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Please note: the above information is subject to change and we endeavour to keep this website up to date wherever necessary.